目录号：ET-500 （500U规格）

目录号：ET-200k （200KU规格）

活性单位：

1单位（U）Taq DNA Polymerase 活性定义为在74℃、30min内，以活性化的大马哈鱼精子DNA作为模板引物，将10 nmol脱氧核苷酸掺入到酸不溶物质所需的酶量。

质量控制：

 SDS-PAGE 检测纯度大于99%，经检测无外源核酸酶活性；PCR方法检测无宿主残余DNA，能有效地扩增人基因组中的单拷贝基因；室温存放一周，无明显活性改变。

酶贮存缓冲液：

20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, 100 mM KCl, Stabilizers, 50% glycerol。

10×Taq Buffer+：200 mM Tris-HCl (pH 8.4)， 200 mM KCl， 100 mM(NH₄)₂SO₄，15 mM MgCl₂。

10×Taq Buffer-：200 mM Tris-HCl (pH 8.4)， 200 mM KCl， 100 mM(NH₄)₂SO₄。

注意：10×Taq Buffer 分为含Mg²⁺和不含Mg²⁺两种，可自选。若不含Mg²⁺的Buffer，另外配有25 mM MgCl₂，如果没有特别指定，通常提供的为含有Mg²⁺的Buffer。

适用范围：

用于DNA片断的PCR扩增、DNA标记、引物延伸、序列测定、平末端加A等，产物可以直接用于TA载体克隆。

储存条件：

-20℃保存

说明书： Taq DNA聚合酶

相关：Taq DNA聚合酶（无甘油），2×Taq PCR MasterMix，Pfu DNA聚合酶

精选引用

对于使用我们的Taq DNA聚合酶的已发表研究感兴趣吗？

Pseudomonas spp. associated with tomato pith necrosis in the Salto area, Northwest Uruguay

^{_{European Journal of Plant Pathology    |     19 Jan 2023    |    DOI: https://doi.org/10.1007/s10658-023-02639-6}}

^{_{The PCR reactions contained 1X PCR buffer, 2.5 mM MgCl2, 0.4 mM of each dNTP, 0.4 μM of each primer, 1 U Taq polymerase (SBS Genetech Co., Ltd., China), and 1 μL of template DNA (100 ng μl−1). The PCR reaction was adjusted to a final volume of 25 μl with MQ water.}}

Sequencing of Norovirus in Southern, Nigeria: Prevalent Genotypes and Putative GII.4 Novel Recombinants among Children

^{_{Genetic Variation    |    16 Dec 2020    |    DOI: https://doi.org/10.5772/intechopen.94389}}

^{_{The RT-PCR used is a very sensitive method, it can detect as few as 5 x 106 copies per gram of stool sample. U-TaQ DNA polymerase (SBS genetech, Beijing, China), a high-fidelity thermostable enzyme that can withstand prolonged incubation at high temperature up to 95°C without significant loss of activity was used for this RT-PCR protocol.}}

Semi-nested polymerase chain reaction over blood culture in detection of bloodstream fungal infection in leukemic children with febrile neutropenia

^{_{Journal of Applied Hematology    |    17 Nov 2020    |    DOI: https://doi.org/10.4103/joah.joah_41_20}}

^{_{Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.}}

Expression, purification and initial characterization of human serum albumin domain I and its cysteine 34

^{_{PLOS ONE    |    12 Oct 2020    |    DOI: https://doi.org/10.1007/s10658-023-02639-6}}

^{_{Total RNA from HepG2 cells was isolated using the RNeasy mini kit (QIAGEN) following manufacturer’s instructions. Retrotranscription was performed using 2.5 μg of RNA, a specific HSA oligonucleotide (ATAAGCCTAAGGCAGCTTGACTGG) and Superscript II reverse transcriptase (Invitrogen). The full-coding sequence of HSA was PCR- amplified with U-Taq (SBS GeneTech)}}

Evaluation of Nested broad-range PCR for Pathogen Detection in Negative Blood Cultures

^{_{JOURNAL OF CLINICAL RESEARCH AND APPLIED MEDICINE   |    6 Oct 2020    |    DOI: https://doi.org/10.5530/jcram.2.2.11}}

^{_{Taq polymerase enzymes and customized primers were procured from SBS Genetech Co., Ltd., China.}}

Identificación por catálogo y detección molecular de bovinos Holstein portadores de braquiespina en Uruguay

^{_{Revista FAVE. Sección Ciencias veterinarias   |    1 Aug 2020    |    DOI: https://doi.org/10.14409/favecv.v19i2.9523}}

^{_{La reacción fue puesta a punto en un volumen final de 25µL conteniendo: 100ng de ADN genómico, 2.5µL de Buffer de PCR 10X (Mg2+: 20mM), 1µM de cada primer, 10mM dNTPs y 0.4µL U-Taq ADN polimerasa (SBS Genetech Co., Ltd., China).}}

仅用于研究，不用于治疗人类或动物

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