CRISPR基因编辑平台

Molecular Therapy | 08 Jan 2025 | DOI: https://doi.org/10.1016/j.ymthe.2024.11.024

sgRNAs for the S. aureus Cas9 system were synthesized by either Synthego or SBS Genetech with 2′-O-methyl and 3′-phosphorothioate on the three terminal nucleotides at both ends

Molecular Medicine | 23 Feb 2024 | DOI: https://doi.org/10.1186/s10020-024-00794-y

The aim was to disrupt the blaNDM-1 Gene in CRKP strains using CRISPR/Cas9 technology. sgRNAs targeting the blaNDM-1 Gene were designed using CRISPOR and synthesized by SBS Genetech.

Science Advances | 7 Feb 2024 | DOI: https://doi.org/10.1126/sciadv.adk2285

Biotin end-labeled probe was prepared by SBS Genetech (China).

Cell Metabolism | 05 Jan 2021 | DOI: https://doi.org/10.1016/j.cmet.2020.12.010

The human CD147 mutants (K234R, K250R, K259R, and K261R) were generated from the eGFP-N1-CD147 plasmid using a site-directed mutagenesis kit (SBS).

Nature Communications | 03 Oct 2017 | DOI: https://doi.org/10.1038/s41467-017-00523-6

To enhance PPARγ (D64A) expression efficiency in PPARγ wild type knockout THP-1 cells, the synonymous mutation on PPARγ coding sequence (gRNA binding site) was generated: (nucleotides 58–72) 5′-GTGGATCTCTCCGTA-3′ mutated to 5′-GTTGATTTGTCCGTG-3′ by using Site-Directed Mutagenesis Kit (FMTG-25, SBS Genetech, Beijing, China).