肽核酸(PNA) IDH1 突变检测试剂盒
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介绍
IDH1(异柠檬酸脱氢酶1,IDH1)突变可在胶质母细胞瘤和骨髓增殖性肿瘤中发现。IDH1突变检测可以作为预后的积极预测因子和胶质母细胞瘤患者的分子标志物。另一方面,据报道,IDH1突变与骨髓增殖性肿瘤的不良预后相关。
总共可以检测和区分 5 种 IDH1 突变
Mutations突变 | Size规格 |
Codon 132 | 25 tests |
5 |
特点
高灵敏度和特异性,即使是少量DNA(1%检测下限,使用10 ng的野生型/突变型混合DNA)
基于实时PCR的即用型试剂盒
短运行时间(3小时内)
流程
样本准备
总DNA提取
实时PCR
结果分析
兼容样本类型
FFPE 组织
新鲜组织
兼容的实时PCR设备
CFX96 (Bio-Rad)
Rotor-Gene Q (Qiagen)
ABI7500 (Thermo Fisher Scientific)
LightCycler 480II (Roche)
精选引用
想了解使用我们的PNA已发表的研究文献吗?
Exponential Increase in an Ionic Signal: A Dominant Role of the Space Charge Effect on the Outer Surface of Nanochannels
Anal. Chem. | 28 Sep 2021 | DOI: https://doi.org/10.1021/acs.analchem.1c03431
All single-stranded PNA sequences were purchased from SBS Genetech Co., Ltd. (Beijing, China).
Peptide Nucleic Acid-Functionalized Nanochannel Biosensor for the Highly Sensitive Detection of Tumor Exosomal MicroRNA
Anal. Chem. | 30 July 2021 | DOI: https://doi.org/10.1021/acs.analchem.1c01898
PNA was synthesized by SBS Genetech Co. Ltd. (Beijing, China). Table S1 shows the above-mentioned oligonucleotide sequence. Sodium hydroxide (NaOH), formic acid (HCOOH), and other chemical reagents were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
Ultrasensitive Exosomal MicroRNA Detection with a Supercharged DNA Framework Nanolabel
Anal. Chem. | 2 April 2021 | DOI: https://doi.org/10.1021/acs.analchem.1c00295
Thiolated PNA with a−(CH2)6− spacer at the 5′ end was synthesized and purified by SBS Genetech Co., Ltd. (Beijing, China). All the sequences
are shown in Table S1.
仅用于研究,不用于治疗人类或动物
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