肽核酸(PNA) c-KIT 突变检测试剂盒
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介绍
C-kit(也称为CD117)可能在某些类型的癌细胞中(包括胃肠道间质瘤和黑色素瘤)以高于正常的量或变化的形式出现。测量肿瘤组织中的C-kit量可能有助于诊断癌症和制定治疗计划。C-kit是一种受体酪氨酸激酶和肿瘤标志物。
总共可以检测到 5 种 c-KIT
Mutations突变 | Size规格 |
Codon 816 | 25 tests |
5 |
特点
高灵敏度和特异性,即使是少量DNA(2%检测下限,使用10 ng的野生型/突变型混合DNA)
基于实时PCR的即用型试剂盒
短运行时间(3小时内)
流程
样本准备
总DNA提取
实时PCR
结果分析
兼容样本类型
FFPE组织
活检组织
兼容的实时PCR设备
CFX96 (Bio-Rad)
QuantStudio5 (Thermo Fisher Scientific)
LightCycler 480II (Roche)
ABI7500 (Thermo Fisher Scientific)
ABI7900HT (Thermo Fisher Scientific)
StepOnePlus (Thermo Fisher Scientific)
Rotor-Gene Q (Qiagen)
精选引用
想了解使用我们的PNA已发表的研究文献吗?
Exponential Increase in an Ionic Signal: A Dominant Role of the Space Charge Effect on the Outer Surface of Nanochannels
Anal. Chem. | 28 Sep 2021 | DOI: https://doi.org/10.1021/acs.analchem.1c03431
All single-stranded PNA sequences were purchased from SBS Genetech Co., Ltd. (Beijing, China).
Peptide Nucleic Acid-Functionalized Nanochannel Biosensor for the Highly Sensitive Detection of Tumor Exosomal MicroRNA
Anal. Chem. | 30 July 2021 | DOI: https://doi.org/10.1021/acs.analchem.1c01898
PNA was synthesized by SBS Genetech Co. Ltd. (Beijing, China). Table S1 shows the above-mentioned oligonucleotide sequence. Sodium hydroxide (NaOH), formic acid (HCOOH), and other chemical reagents were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
Ultrasensitive Exosomal MicroRNA Detection with a Supercharged DNA Framework Nanolabel
Anal. Chem. | 2 April 2021 | DOI: https://doi.org/10.1021/acs.analchem.1c00295
Thiolated PNA with a−(CH2)6− spacer at the 5′ end was synthesized and purified by SBS Genetech Co., Ltd. (Beijing, China). All the sequences
are shown in Table S1.
仅用于研究,不用于治疗人类或动物
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