Cas9 Nuclease is a recombinant Streptococcus pyogenes Cas9 (wt) protein. A nucleic localization signal (NLS) is added which can be used for genome editing by inducing site-specific DNA double-stranded breaks. Cas9 protein can form ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS.
Our CRISPR-dCas9 Nuclese is from Streptococcus pyogenes. The PAM sequence of dCas9 is 5-NGG, which is highly abundant in the genome. dCas9 protein is a ribozyme deficient Cas9 protein, which does not have sgRNA guided DNA strand cleavage activity, but retains target DNA binding activity.
LwCas13a, belonging to the class II type VI CRISPR system, contains two higher eukaryotes and prokaryotes nucleoside binding domains. It has been confirmed that LwCas13a is a novel CRISPR nuclease with RNA-targeted cleavage activity.
SpRYCas9 is an RNA-mediated nuclease, which can catalyze the specific site cleavage of double-stranded DNA. It can recognize NNN (NRN > NYN) PAM sequence, which almost gets rid of the restriction of the PAM.